Sensory Evaluation of Chickpea Aquafaba as an Egg Substitute in Vegan Cupcake Formulation

Authors

  • Gülsüm AKPINAR
  • İbrahim Tuğkan ŞEKER

DOI:

https://doi.org/10.63556/jotags.2026.1804

Keywords:

Aquafaba, Vegan, Sensory evaluation, Cupcakes

Abstract

This study aimed to investigate the use of aquafaba, a plant-based ingredient, as an alternative to eggs in cupcake production. Two formulations, traditional and vegan, were prepared and their sensory properties were comparatively evaluated. Sensory analysis was carried out with 30 semi-trained panelists in two replicates, using difference-from-control, scoring, and hedonic scale tests. In the difference-from-control test, the vegan cupcake was perceived as moderately different from the control. According to the scoring test, statistically significant differences were found in appearance attributes, as the vegan cupcake was rated with a darker crust color and a less uniform crumb structure, while no significant differences were observed in aroma, texture, or flavor attributes. In the hedonic scale test, both samples received high overall liking scores, with the vegan cupcake receiving slightly higher ratings than the traditional one. Overall, these findings suggest that aquafaba presents a favorable sensory profile and holds potential as a viable egg substitute in cupcake formulations.

References

Alsalman, F., Tulbek, M. C., Nickerson, M., & Ramaswamy, H. S. (2020). Evaluation and optimization of functional and antinutritional properties of aquafaba. Legume Science, 2(2), e30. doi:10.1002/leg3.30

Alsalman, F. B., & Ramaswamy, H. S. (2021). Evaluation of changes in protein quality of high-pressure treated aqueous aquafaba. Molecules, 26(1), 234. doi:10.3390/molecules26010234

Aslan, M., & Ertaş, N. (2020). Possibility of using ‘chickpea aquafaba’ as egg replacer in traditional cake formulation. Harran Journal of Agricultural and Food Science, 24(1), 1–8. doi:10.29050/harranziraat.569397

Bakaloudi, D. R., Halloran, A., Rippin, H. L., Oikonomidou, A. C., Dardavesis, T. I., Williams, J., Wickramasinghe, K., Breda, J., & Chourdakis, M. (2021). Intake and adequacy of the vegan diet: A systematic review of the evidence. Clinical Nutrition, 40(5), 3503–3521. doi:10.1016/j.clnu.2020.11.035

Bird, L. G., Pilkington, C. L., Saputra, A., & Serventi, L. (2017). Products of chickpea processing as texture improvers in gluten-free bread. Food Science and Technology International, 23(8), 690–698. doi:10.1177/1082013217717802

Buhl, T. F., Christensen, C. H., & Hammershøj, M. (2019). Aquafaba as an egg white substitute in food foams and emulsions: Protein composition and functional behavior. Food Hydrocolloids, 96, 354–364. doi:10.1016/j.foodhyd.2019.05.041

Carpenter, R. P., Lyon, D. H., & Hasdell, T. A. (2000). Guidelines for sensory analysis in food product development and quality control (2nd ed.). Gaithersburg, MD: Aspen Publishers.

Crawford, K., Tyl, C., & Kerr, W. (2023). Evaluation of processing conditions and hydrocolloid addition on functional properties of aquafaba. Foods, 12(4), 775. doi:10.3390/foods12040775

De Barros Miranda, B., Silva Holanda, G., Raposo, A., da Costa Maynard, D., Braz Assunção Botelho, R., Romão, B., Ruffo de Oliveira, V., & Puppin Zandonadi, R. (2024). Chickpea aquafaba: A systematic review of the different processes for obtaining and their nutritional and technological characteristics. Journal of Food Science and Technology, 61(8), 1439–1456. doi:10.1007/s13197-023-05920-y

Dinu, M., Abbate, R., Gensini, G. F., Casini, A., & Sofi, F. (2017). Vegetarian, vegan diets and multiple health outcomes: A systematic review with meta-analysis of observational studies. Critical Reviews in Food Science and Nutrition, 57(17), 3640–3649. doi:10.1080/10408398.2016.1138447

Echeverria-Jaramillo, E., & Shin, W.-S. (2023). Current processing methods of aquafaba. Trends in Food Science & Technology, 138, 441–452. doi:10.1016/j.tifs.2023.06.022

Fehér, A., Gazdecki, M., Véha, M., Szakály, M., & Szakály, Z. (2020). A comprehensive review of the benefits of and the barriers to the switch to a plant-based diet. Sustainability, 12(10), 4136. doi:10.3390/su12104136

Fuentes, C., & Fuentes, M. (2017). Making a market for alternatives: Marketing devices and the qualification of a vegan milk substitute. Journal of Marketing Management, 33(3–4), 529–555. doi:10.1080/0267257X.2017.1328456

Gallagher, C. T., Hanley, P., & Lane, K. E. (2022). Pattern analysis of vegan eating reveals healthy and unhealthy patterns within the vegan diet. Public Health Nutrition, 25(5), 1310–1320. doi:10.1017/S136898002100197X

George, D., & Mallery, P. (2020). IBM SPSS statistics 26 step by step: A simple guide and reference. New York, NY: Routledge.

Ghaffari, M., Rodrigo, P. G. K., Ekinci, Y., & Pino, G. (2022). Consumers’ motivations for adopting a vegan diet: A mixed-methods approach. International Journal of Consumer Studies, 46(4), 1193–1208. doi:10.1111/ijcs.12752

Grand View Research. (2022). Vegan food market size, share and trends report. Retrieved September 27, 2025, from https://www.grandviewresearch.com/industry-analysis/vegan-food-market

He, Y., Shim, Y. Y., Mustafa, R., Meda, V., & Reaney, M. J. T. (2019). Chickpea cultivar selection to produce aquafaba with superior emulsion properties. Foods, 8(12), 685. doi:10.3390/foods8120685

He, Y., Meda, V., Reaney, M. J. T., & Mustafa, R. (2021). Aquafaba, a new plant-based rheological additive for food applications. Trends in Food Science & Technology, 111, 27–47. doi:10.1016/j.tifs.2021.02.035

Janssen, M., Busch, C., Rödiger, M., & Hamm, U. (2016). Motives of consumers following a vegan diet and their attitudes towards animal agriculture. Appetite, 105, 643–651. doi:10.1016/j.appet.2016.06.039

Kablan, H., Artık, N., Karasu, S., Tekin, Z. H., Sağdıç, O., & Cankurt, H. (2022). The effect of gypsophila extract as natural emulsifier on the steady, dynamic rheological behavior and microstructural properties of the ice cream mix. European Journal of Science and Technology, 38, 1–7. doi:10.31590/ejosat.1086551

Koeder, C., & Perez-Cueto, F. J. A. (2022). Vegan nutrition: A preliminary guide for health professionals. Critical Reviews in Food Science and Nutrition, 64(3), 670–707. doi:10.1080/10408398.2022.2107997

Konal, G., Dundar, A. N., Sahin, O. I., Parlak, M. E., & Saricaoglu, F. T. (2025). Characterization of cakes produced with different legume aquafaba. International Journal of Gastronomy and Food Science, 39, 101076. doi:10.1016/j.ijgfs.2024.101076

Lafarga, T., Villaró, S., Bobo, G., & Aguiló-Aguayo, I. (2019). Optimisation of the pH and boiling conditions needed to obtain improved foaming and emulsifying properties of chickpea aquafaba using a response surface methodology. International Journal of Gastronomy and Food Science, 18, 100177. doi:10.1016/j.ijgfs.2019.100177

Lima, L. T., Zandonadi, R. P., Rodrigues, G., Aguiar, K., Romão, B., Mendonça, M., Raposo, A., Teixeira-Lemos, E., & Botelho, R. B. A. (2024). Chickpea aquafaba production techniques for foaming: A comparison of foam stability considering the use of soaking water, additives, pressure cooking time, pH, and protein content. LWT - Food Science and Technology, 207, 116643. doi:10.1016/j.lwt.2024.116643

Martinelli, E., & De Canio, F. (2022). Non-vegan consumers buying vegan food: The moderating role of conformity. British Food Journal, 124(1), 14–30. doi:10.1108/BFJ-01-2021-0023

Meurer, M. C., de Souza, D., & Marczak, L. D. F. (2020). Effects of ultrasound on technological properties of chickpea cooking water (aquafaba). Journal of Food Engineering, 265, 109688. doi:10.1016/j.jfoodeng.2019.109688

Mustafa, R., He, Y., Shim, Y. Y., & Reaney, M. J. T. (2018). Aquafaba, wastewater from chickpea canning, functions as an egg replacer in sponge cake. International Journal of Food Science & Technology, 53(10), 2247–2255. doi:10.1111/ijfs.13813

Mustafa, R., & Reaney, M. J. T. (2020). Aquafaba, from food waste to a value-added product. In R. Campos-Vega, B. D. Oomah, & H. A. Vergara-Castañeda (Eds.), Food wastes and by-products: Nutraceutical and health potential (pp. 93–126). Wiley-Blackwell. doi:10.1002/9781119534167.ch4

Onoğur, T. A., & Elmacı, Y. (2019). Gıdalarda duyusal değerlendirme (4th ed.). İzmir: Sidas Medya.

Ozcan, I., Ozyigit, E., Erkoc, S., Tavman, S., & Kumcuoglu, S. (2023). Investigating the physical and quality characteristics and rheology of mayonnaise containing aquafaba as an egg substitute. Journal of Food Engineering, 344, 111388. doi:10.1016/j.jfoodeng.2022.111388

Pointke, M., & Pawelzik, E. (2022). Plant-based alternative products: Are they healthy alternatives? Micro- and macronutrients and nutritional scoring. Nutrients, 14(3), 601. doi:10.3390/nu14030601

Qian, F., Liu, G., Hu, F. B., Bhupathiraju, S. N., & Sun, Q. (2019). Association between plant-based dietary patterns and risk of type 2 diabetes: A systematic review and meta-analysis. JAMA Internal Medicine, 179(10), 1335–1344. doi:10.1001/jamainternmed.2019.2195

Raikos, V., Hayes, H. E., & Ni, H. (2020). Aquafaba from commercially canned chickpeas as potential egg replacer for the development of vegan mayonnaise: Recipe optimisation and storage stability. International Journal of Food Science and Technology, 55(5), 1935–1942. doi:10.1111/ijfs.14427

Rogers, L. (2010). Sensory methods for quality control. In D. Kilcast (Ed.), Sensory analysis for food and beverage quality control: A practical guide (pp. 51–74). Cambridge: Woodhead Publishing.

Roessel, J. (2014). Révolution végétale. Retrieved September 28, 2025, from http://www.revolutionvegetale.com/en

Roozen, M., & Serventi, L. (2022). Ingredients from climate resilient crops to enhance the nutritional quality of gluten-free bread. Foods, 11(11), 1628. doi:10.3390/foods11111628

Selinger, E., Neuenschwander, M., Koller, A., Gojda, J., Kühn, T., Schwingshackl, L., Barbaresko, J., & Schlesinger, S. (2023). Evidence of a vegan diet for health benefits and risks – an umbrella review of meta-analyses of observational and clinical studies. Critical Reviews in Food Science and Nutrition, 63(29), 9926–9936. doi:10.1080/10408398.2022.2075311

Shim, Y. Y., Mustafa, R., Shen, J., Ratanapariyanuch, K., & Reaney, M. J. T. (2018). Composition and properties of aquafaba: Water recovered from commercially canned chickpeas. Journal of Visualized Experiments, 132, e56305. doi:10.3791/56305

Stantiall, S., Dale, K. J., Calizo, F. S., & Serventi, L. (2018). Application of pulses cooking water as functional ingredients: The foaming and gelling abilities. European Food Research and Technology, 244(11), 97–104. doi:10.1007/s00217-017-2943-x

Stasiak, J., Stasiak, D. M., & Libera, J. (2023). The potential of aquafaba as a structure-shaping additive in plant-derived food technology. Applied Sciences, 13(7), 4122. doi:10.3390/app13074122

Yazici, G. N., Taspinar, T., Binokay, H., Dagsuyu, C., Kokangul, A., & Ozer, M. S. (2023). Investigating the potential of using aquafaba in eggless gluten-free cake production by multicriteria decision-making approach. Journal of Food Measurement and Characterization, 17(4), 5759–5776. doi:10.1007/s11694-023-

Yazici, G. N., Taspinar, T., Binokay, H., Agcam, E., Agirman, B., & Ozer, M. S. (2025). Assessment of physicochemical properties and staling characteristics of eggless gluten-free cakes with aquafaba. Journal of Food Measurement and Characterization, 19(4), 2557–2573. doi:10.1007/s11694-025-03131-x

Published

30-03-2026

How to Cite

AKPINAR , G., & ŞEKER , İbrahim T. (2026). Sensory Evaluation of Chickpea Aquafaba as an Egg Substitute in Vegan Cupcake Formulation. Journal of Tourism & Gastronomy Studies, 14(1), 536–546. https://doi.org/10.63556/jotags.2026.1804

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